| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Walker Zimmerman | Defender - Centre-Back | United States | Nashville SC | 1993 | €2.5m |
| 2 |  Sverrir Ingi Ingason | Defender - Centre-Back | Iceland | Panathinaikos FC | 1993 | €2.5m |
| 3 |  Jubal | Defender - Centre-Back | Brazil | FC Krasnodar | 1993 | €2.5m |
| 4 |  Jesús Murillo | Defender - Centre-Back | Colombia | FC Juárez | 1994 | €2.3m |
| 5 |  Conor Coady | Defender - Centre-Back | England | Wrexham AFC | 1993 | €2.2m |
| 6 |  Connor Goldson | Defender - Centre-Back | England | Aris Limassol | 1992 | €2.8m |
| 7 |  Alexis Pérez | Defender - Centre-Back | Colombia | Without Club | 1994 | €2.2m |
| 8 |  Júnior Alonso | Defender - Centre-Back | Paraguay | Clube Atlético Mineiro | 1993 | €3.0m |
| 9 |  Eric Bailly | Defender - Centre-Back | Cote d'Ivoire | Real Oviedo | 1994 | €2.0m |
| 10 |  Unai Bilbao | Defender - Centre-Back | Spain | Club Tijuana | 1994 | €2.0m |
| 11 |  Aritz Elustondo | Defender - Centre-Back | Spain | Real Sociedad | 1994 | €3.0m |
| 12 |  Georgiy Dzhikiya | Defender - Centre-Back | Russia | Antalyaspor | 1993 | €2.0m |
| 13 |  Edoardo Goldaniga | Defender - Centre-Back | Italy | Como 1907 | 1993 | €3.0m |
| 14 |  Rodrigo Ely | Defender - Centre-Back | Brazil Italy | Grêmio Foot-Ball Porto Alegrense | 1993 | €2.0m |
| 15 |  Lautaro Giannetti | Defender - Centre-Back | Argentina Italy | Antalyaspor | 1993 | €2.0m |
| 16 |  Jannik Vestergaard | Defender - Centre-Back | Denmark Germany | Leicester City | 1992 | €2.0m |
| 17 |  Robin Pröpper | Defender - Centre-Back | Netherlands | Twente Enschede FC | 1993 | €2.0m |
| 18 |  Omar Colley | Defender - Centre-Back | The Gambia | Al-Diraiyah FC | 1992 | €2.0m |
| 19 |  Samuel Gigot | Defender - Centre-Back | France | SS Lazio | 1993 | €3.0m |
| 20 |  Mattia Bani | Defender - Centre-Back | Italy | Palermo FC | 1993 | €1.8m |
| 21 |  Wesley Hoedt | Defender - Centre-Back | Netherlands | Al-Shabab FC | 1994 | €1.8m |
| 22 |  Andreas Maxsø | Defender - Centre-Back | Denmark | Colorado Rapids | 1994 | €1.8m |
| 23 |  Yeimar Gómez Andrade | Defender - Centre-Back | Colombia | Seattle Sounders FC | 1992 | €1.8m |
| 24 |  Seung-hyun Jung | Defender - Centre-Back | Korea, South | Ulsan HD FC | 1994 | €1.6m |
| 25 |  Luan | Defender - Centre-Back | Brazil | Deportivo Toluca | 1993 | €1.5m |
| 26 |  Julio Cascante | Defender - Centre-Back | Costa Rica | Austin FC | 1993 | €1.5m |
| 27 |  Adam Masina | Defender - Centre-Back | Morocco Italy | Torino FC | 1994 | €1.5m |
| 28 |  William Troost-Ekong | Defender - Centre-Back | Nigeria Netherlands | Al-Kholood Club | 1993 | €1.5m |
| 29 |  Rúben Semedo | Defender - Centre-Back | Portugal Cape Verde | Without Club | 1994 | €1.5m |
| 30 |  Samet Akaydin | Defender - Centre-Back | Türkiye | Caykur Rizespor | 1994 | €1.5m |