| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Tyrone Mings | Defender - Centre-Back | England | Aston Villa | 1993 | €5.0m |
| 2 |  Michael Keane | Defender - Centre-Back | England Ireland | Everton FC | 1993 | €5.0m |
| 3 |  Ben Davies | Defender - Centre-Back | Wales | Tottenham Hotspur | 1993 | €6.0m |
| 4 |  Berat Djimsiti | Defender - Centre-Back | Albania Switzerland | Atalanta BC | 1993 | €6.0m |
| 5 |  Jonathan Gradit | Defender - Centre-Back | France | RC Lens | 1992 | €4.0m |
| 6 |  Diego Llorente | Defender - Centre-Back | Spain | Real Betis Balompié | 1993 | €6.0m |
| 7 |  Dan Burn | Defender - Centre-Back | England | Newcastle United | 1992 | €6.0m |
| 8 |  David Alaba | Defender - Centre-Back | Austria | Real Madrid | 1992 | €6.0m |
| 9 |  Jamaal Lascelles | Defender - Centre-Back | England Barbados | Newcastle United | 1993 | €4.0m |
| 10 |  Brandon Mechele | Defender - Centre-Back | Belgium | Club Brugge KV | 1993 | €3.5m |
| 11 |  Pablo Marí | Defender - Centre-Back | Spain | ACF Fiorentina | 1993 | €3.5m |
| 12 |  Clinton Mata | Defender - Centre-Back | Angola Belgium | Olympique Lyon | 1992 | €3.5m |
| 13 |  Dominik Kohr | Defender - Centre-Back | Germany | 1.FSV Mainz 05 | 1994 | €3.5m |
| 14 |  Jakob Glesnes | Defender - Centre-Back | Norway | Philadelphia Union | 1994 | €3.5m |
| 15 |  Ethan Pinnock | Defender - Centre-Back | Jamaica England | Brentford FC | 1993 | €7.0m |
| 16 |  Matthias Ginter | Defender - Centre-Back | Germany | SC Freiburg | 1994 | €7.0m |
| 17 |  David García | Defender - Centre-Back | Spain | Al-Rayyan SC | 1994 | €7.0m |
| 18 |  Fábio Cardoso | Defender - Centre-Back | Portugal | Sevilla FC | 1994 | €3.0m |
| 19 |  Edoardo Goldaniga | Defender - Centre-Back | Italy | Como 1907 | 1993 | €3.0m |
| 20 |  Samuel Gigot | Defender - Centre-Back | France | SS Lazio | 1993 | €3.0m |
| 21 |  Aritz Elustondo | Defender - Centre-Back | Spain | Real Sociedad | 1994 | €3.0m |
| 22 |  Sead Kolasinac | Defender - Centre-Back | Bosnia-Herzegovina Germany | Atalanta BC | 1993 | €7.0m |
| 23 |  Júnior Alonso | Defender - Centre-Back | Paraguay | Clube Atlético Mineiro | 1993 | €3.0m |
| 24 |  Emre Can | Defender - Centre-Back | Germany Türkiye | Borussia Dortmund | 1994 | €7.0m |
| 25 |  Connor Goldson | Defender - Centre-Back | England | Aris Limassol | 1992 | €2.8m |
| 26 |  Jubal | Defender - Centre-Back | Brazil | FC Krasnodar | 1993 | €2.5m |
| 27 |  Patric | Defender - Centre-Back | Spain | SS Lazio | 1993 | €2.5m |
| 28 |  Sverrir Ingi Ingason | Defender - Centre-Back | Iceland | Panathinaikos FC | 1993 | €2.5m |
| 29 |  Walker Zimmerman | Defender - Centre-Back | United States | Nashville SC | 1993 | €2.5m |
| 30 |  Jesús Murillo | Defender - Centre-Back | Colombia | FC Juárez | 1994 | €2.3m |