| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Léo Pereira | Defender - Centre-Back | Brazil | CR Flamengo | 1996 | €9.0m |
| 2 |  Tsuyoshi Watanabe | Defender - Centre-Back | Japan | Feyenoord Rotterdam | 1997 | €9.0m |
| 3 |  Niklas Süle | Defender - Centre-Back | Germany | Borussia Dortmund | 1995 | €8.0m |
| 4 |  Lukas Klostermann | Defender - Centre-Back | Germany | RB Leipzig | 1996 | €8.0m |
| 5 |  Nico Elvedi | Defender - Centre-Back | Switzerland | Borussia Mönchengladbach | 1996 | €8.0m |
| 6 |  Fabrício Bruno | Defender - Centre-Back | Brazil | Cruzeiro Esporte Clube | 1996 | €10.0m |
| 7 |  Omar Alderete | Defender - Centre-Back | Paraguay | Sunderland AFC | 1996 | €8.0m |
| 8 |  Clément Lenglet | Defender - Centre-Back | France | Atlético de Madrid | 1995 | €7.5m |
| 9 |  Vítor Tormena | Defender - Centre-Back | Brazil | FC Krasnodar | 1996 | €7.0m |
| 10 |  Lucas Martínez Quarta | Defender - Centre-Back | Argentina Italy | CA River Plate | 1996 | €7.0m |
| 11 |  Mario Hermoso | Defender - Centre-Back | Spain | AS Roma | 1995 | €7.0m |
| 12 |  Duje Caleta-Car | Defender - Centre-Back | Croatia | Real Sociedad | 1996 | €7.0m |
| 13 |  Denis Vavro | Defender - Centre-Back | Slovakia | VfL Wolfsburg | 1996 | €7.0m |
| 14 |  Nino | Defender - Centre-Back | Brazil | Zenit St. Petersburg | 1997 | €11.0m |
| 15 |  Srdjan Babic | Defender - Centre-Back | Serbia Bosnia-Herzegovina | Spartak Moscow | 1996 | €6.0m |
| 16 |  Murilo | Defender - Centre-Back | Brazil | Sociedade Esportiva Palmeiras | 1997 | €6.0m |
| 17 |  Ardian Ismajli | Defender - Centre-Back | Albania Kosovo | Torino FC | 1996 | €6.0m |
| 18 |  Carl Starfelt | Defender - Centre-Back | Sweden | Celta de Vigo | 1995 | €6.0m |
| 19 |  Jeremiah St. Juste | Defender - Centre-Back | Netherlands St. Kitts & Nevis | Sporting CP B | 1996 | €6.0m |
| 20 |  Martin Valjent | Defender - Centre-Back | Slovakia | RCD Mallorca | 1995 | €6.0m |
| 21 |  Igor Zubeldia | Defender - Centre-Back | Spain | Real Sociedad | 1997 | €12.0m |
| 22 |  Lyanco | Defender - Centre-Back | Brazil Serbia | Clube Atlético Mineiro | 1997 | €6.0m |
| 23 |  Philipp Lienhart | Defender - Centre-Back | Austria | SC Freiburg | 1996 | €12.0m |
| 24 |  Andreas Christensen | Defender - Centre-Back | Denmark | FC Barcelona | 1996 | €12.0m |
| 25 |  Ko Itakura | Defender - Centre-Back | Japan | Ajax Amsterdam | 1997 | €12.0m |
| 26 |  Lucas Veríssimo | Defender - Centre-Back | Brazil | Al-Wakrah SC | 1995 | €5.5m |
| 27 |  Bernardo | Defender - Centre-Back | Brazil | TSG 1899 Hoffenheim | 1995 | €5.5m |
| 28 |  Joe Worrall | Defender - Centre-Back | England | Burnley FC | 1997 | €5.0m |
| 29 |  Luiz Felipe | Defender - Centre-Back | Italy Brazil | Rayo Vallecano | 1997 | €5.0m |
| 30 |  Kevin Diks | Defender - Centre-Back | Indonesia Netherlands | Borussia Mönchengladbach | 1996 | €5.0m |