| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Thiago Martins | Defender - Centre-Back | Brazil | New York City FC | 1995 | €2.5m |
| 2 |  Joel Waterman | Defender - Centre-Back | Canada | Chicago Fire FC | 1996 | €2.5m |
| 3 |  Lucas Merolla | Defender - Centre-Back | Argentina | Mazatlán FC | 1995 | €2.5m |
| 4 |  Jack Elliott | Defender - Centre-Back | England Scotland | Chicago Fire FC | 1995 | €2.5m |
| 5 |  Daniel Amartey | Defender - Centre-Back | Ghana | Without Club | 1994 | €2.5m |
| 6 |  Paweł Dawidowicz | Defender - Centre-Back | Poland | Without Club | 1995 | €2.5m |
| 7 |  Kevin Akpoguma | Defender - Centre-Back | Nigeria Germany | TSG 1899 Hoffenheim | 1995 | €2.5m |
| 8 |  Ben Davies | Defender - Centre-Back | England | Oxford United | 1995 | €2.5m |
| 9 |  Pawel Bochniewicz | Defender - Centre-Back | Poland | SC Heerenveen | 1996 | €2.5m |
| 10 |  Stiven Barreiro | Defender - Centre-Back | Colombia | Club León FC | 1994 | €2.5m |
| 11 |  Tin Jedvaj | Defender - Centre-Back | Croatia | Panathinaikos FC | 1995 | €2.7m |
| 12 |  Rubén Duarte | Defender - Centre-Back | Spain | UNAM Pumas | 1995 | €2.8m |
| 13 |  Rob Dickie | Defender - Centre-Back | England | Bristol City | 1996 | €2.8m |
| 14 |  Matt Miazga | Defender - Centre-Back | United States Poland | FC Cincinnati | 1995 | €2.2m |
| 15 |  Ousseynou Ba | Defender - Centre-Back | Senegal | Basaksehir FK | 1995 | €2.0m |
| 16 |  Dominic Hyam | Defender - Centre-Back | Scotland England | Wrexham AFC | 1995 | €2.0m |
| 17 |  Cameron Burgess | Defender - Centre-Back | Australia Scotland | Swansea City | 1995 | €2.0m |
| 18 |  Chancel Mbemba | Defender - Centre-Back | DR Congo | LOSC Lille | 1994 | €2.0m |
| 19 |  Milos Veljkovic | Defender - Centre-Back | Serbia Switzerland | Red Star Belgrade | 1995 | €3.0m |
| 20 |  Mauricio Lemos | Defender - Centre-Back | Uruguay | Clube de Regatas Vasco da Gama | 1995 | €2.0m |
| 21 |  Paddy McNair | Defender - Centre-Back | Northern Ireland | San Diego FC | 1995 | €3.0m |
| 22 |  Jake Cooper | Defender - Centre-Back | England | Millwall FC | 1995 | €2.0m |
| 23 |  Marc Oliver Kempf | Defender - Centre-Back | Germany | Como 1907 | 1995 | €3.0m |
| 24 |  Luis Abram | Defender - Centre-Back | Peru Italy | Club Sporting Cristal | 1996 | €2.0m |
| 25 |  Vaclav Jemelka | Defender - Centre-Back | Czech Republic | FC Viktoria Plzen | 1995 | €2.0m |
| 26 |  Stian Gregersen | Defender - Centre-Back | Norway | Atlanta United FC | 1995 | €2.0m |
| 27 |  Patrick Mainka | Defender - Centre-Back | Germany | 1.FC Heidenheim 1846 | 1994 | €3.0m |
| 28 |  Benjamín Kuscevic | Defender - Centre-Back | Chile Croatia | Fortaleza Esporte Clube | 1996 | €2.0m |
| 29 |  Jason Denayer | Defender - Centre-Back | Belgium DR Congo | Without Club | 1995 | €2.0m |
| 30 |  Daniele Rugani | Defender - Centre-Back | Italy | Juventus FC | 1994 | €3.0m |