| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Lucas Veríssimo | Defender - Centre-Back | Brazil | Al-Wakrah SC | 1995 | €5.5m |
| 2 |  Presnel Kimpembe | Defender - Centre-Back | France DR Congo | Qatar SC | 1995 | €5.0m |
| 3 |  Alexander Barboza | Defender - Centre-Back | Argentina Uruguay | Botafogo de Futebol e Regatas | 1995 | €5.0m |
| 4 |  Srdjan Babic | Defender - Centre-Back | Serbia Bosnia-Herzegovina | Spartak Moscow | 1996 | €6.0m |
| 5 |  Abdou Diallo | Defender - Centre-Back | Senegal France | Al-Duhail SC | 1996 | €5.0m |
| 6 |  Maksim Osipenko | Defender - Centre-Back | Russia | Dynamo Moscow | 1994 | €5.0m |
| 7 |  Willyan Rocha | Defender - Centre-Back | Brazil Italy | Al-Jazira Club | 1995 | €6.0m |
| 8 |  Carl Starfelt | Defender - Centre-Back | Sweden | Celta de Vigo | 1995 | €6.0m |
| 9 |  Martin Valjent | Defender - Centre-Back | Slovakia | RCD Mallorca | 1995 | €6.0m |
| 10 |  Brendan Chardonnet | Defender - Centre-Back | France | Stade Brestois 29 | 1994 | €6.0m |
| 11 |  Lucas Martínez Quarta | Defender - Centre-Back | Argentina Italy | CA River Plate | 1996 | €7.0m |
| 12 |  Niklas Stark | Defender - Centre-Back | Germany | SV Werder Bremen | 1995 | €4.0m |
| 13 |  Vítor Tormena | Defender - Centre-Back | Brazil | FC Krasnodar | 1996 | €7.0m |
| 14 |  Denis Vavro | Defender - Centre-Back | Slovakia | VfL Wolfsburg | 1996 | €7.0m |
| 15 |  Abdülkerim Bardakcı | Defender - Centre-Back | Türkiye | Galatasaray | 1994 | €7.0m |
| 16 |  Yeray Álvarez | Defender - Centre-Back | Spain | Athletic Bilbao | 1995 | €7.0m |
| 17 |  Mario Hermoso | Defender - Centre-Back | Spain | AS Roma | 1995 | €7.0m |
| 18 |  Clément Lenglet | Defender - Centre-Back | France | Atlético de Madrid | 1995 | €7.5m |
| 19 |  Jean-Charles Castelletto | Defender - Centre-Back | Cameroon France | Al-Duhail SC | 1995 | €3.5m |
| 20 |  Marvin Friedrich | Defender - Centre-Back | Germany | Borussia Mönchengladbach | 1995 | €3.5m |
| 21 |  Gregory Wüthrich | Defender - Centre-Back | Switzerland Ghana | BSC Young Boys | 1994 | €3.5m |
| 22 |  Pedro Henrique | Defender - Centre-Back | Brazil | Red Bull Bragantino | 1995 | €3.5m |
| 23 |  Rob Holding | Defender - Centre-Back | England | Colorado Rapids | 1995 | €3.5m |
| 24 |  Rodrigão | Defender - Centre-Back | Brazil | Red Star Belgrade | 1995 | €3.0m |
| 25 |  Ibrahim Cissé | Defender - Centre-Back | France Senegal | Ferencvárosi TC | 1996 | €3.0m |
| 26 |  Oumar Sako | Defender - Centre-Back | Niger Cote d'Ivoire | FC Rostov | 1996 | €3.0m |
| 27 |  Paddy McNair | Defender - Centre-Back | Northern Ireland | San Diego FC | 1995 | €3.0m |
| 28 |  Alexis Peña | Defender - Centre-Back | Mexico | Club Necaxa | 1996 | €3.0m |
| 29 |  Joyskim Dawa | Defender - Centre-Back | Cameroon France | FCSB | 1996 | €3.0m |
| 30 |  Daniele Rugani | Defender - Centre-Back | Italy | Juventus FC | 1994 | €3.0m |