| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Pascal Struijk | Defender - Centre-Back | Netherlands Belgium | Leeds United | 1999 | €18.0m |
| 2 |  Eric García | Defender - Centre-Back | Spain | FC Barcelona | 2001 | €18.0m |
| 3 |  Jacob Greaves | Defender - Centre-Back | England | Ipswich Town | 2000 | €18.0m |
| 4 |  Anel Ahmedhodzic | Defender - Centre-Back | Bosnia-Herzegovina Sweden | Feyenoord Rotterdam | 1999 | €18.0m |
| 5 |  Alexsandro | Defender - Centre-Back | Brazil | LOSC Lille | 1999 | €20.0m |
| 6 |  Ethan Ampadu | Defender - Centre-Back | Wales England | Leeds United | 2000 | €20.0m |
| 7 |  Tuta | Defender - Centre-Back | Brazil | Al-Duhail SC | 1999 | €15.0m |
| 8 |  Chris Richards | Defender - Centre-Back | United States | Crystal Palace | 2000 | €14.0m |
| 9 |  Dara O'Shea | Defender - Centre-Back | Ireland | Ipswich Town | 1999 | €14.0m |
| 10 |  Matteo Gabbia | Defender - Centre-Back | Italy | AC Milan | 1999 | €14.0m |
| 11 |  Aitor Paredes | Defender - Centre-Back | Spain | Athletic Bilbao | 2000 | €22.0m |
| 12 |  Mohamed Simakan | Defender - Centre-Back | France Guinea | Al-Nassr FC | 2000 | €23.0m |
| 13 |  Nehuén Pérez | Defender - Centre-Back | Argentina | FC Porto | 2000 | €13.0m |
| 14 |  Mohammed Salisu | Defender - Centre-Back | Ghana | AS Monaco | 1999 | €12.0m |
| 15 |  Arthur Theate | Defender - Centre-Back | Belgium | Eintracht Frankfurt | 2000 | €24.0m |
| 16 |  Josip Sutalo | Defender - Centre-Back | Croatia | Ajax Amsterdam | 2000 | €24.0m |
| 17 |  Vitão | Defender - Centre-Back | Brazil | Sport Club Internacional | 2000 | €12.0m |
| 18 |  Luca Ranieri | Defender - Centre-Back | Italy | ACF Fiorentina | 1999 | €12.0m |
| 19 |  Trevoh Chalobah | Defender - Centre-Back | England Sierra Leone | Chelsea FC | 1999 | €25.0m |
| 20 |  Calvin Bassey | Defender - Centre-Back | Nigeria England | Fulham FC | 1999 | €25.0m |
| 21 |  Wesley Fofana | Defender - Centre-Back | France Cote d'Ivoire | Chelsea FC | 2000 | €25.0m |
| 22 |  Facundo Medina | Defender - Centre-Back | Argentina | Olympique Marseille | 1999 | €25.0m |
| 23 |  Hiroki Ito | Defender - Centre-Back | Japan | Bayern Munich | 1999 | €25.0m |
| 24 |  Maxence Lacroix | Defender - Centre-Back | France Guadeloupe | Crystal Palace | 2000 | €25.0m |
| 25 |  Loïc Badé | Defender - Centre-Back | France Cote d'Ivoire | Bayer 04 Leverkusen | 2000 | €25.0m |
| 26 |  Igoh Ogbu | Defender - Centre-Back | Nigeria | SK Slavia Prague | 2000 | €11.0m |
| 27 |  Jean-Clair Todibo | Defender - Centre-Back | France French Guiana | West Ham United | 1999 | €25.0m |
| 28 |  Odilon Kossounou | Defender - Centre-Back | Cote d'Ivoire | Atalanta BC | 2001 | €25.0m |
| 29 |  Leo Østigård | Defender - Centre-Back | Norway | Genoa CFC | 1999 | €10.0m |
| 30 |  David Carmo | Defender - Centre-Back | Angola Portugal | Real Oviedo | 1999 | €10.0m |