| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Sebastián Cáceres | Defender - Centre-Back | Uruguay | CF América | 1999 | €5.0m |
| 2 |  Jorge Cuenca | Defender - Centre-Back | Spain | Fulham FC | 1999 | €5.0m |
| 3 |  Perr Schuurs | Defender - Centre-Back | Netherlands | Torino FC | 1999 | €5.0m |
| 4 |  Mads Bech Sørensen | Defender - Centre-Back | Denmark | FC Midtjylland | 1999 | €5.0m |
| 5 |  Serge-Philippe Raux-Yao | Defender - Centre-Back | France Cote d'Ivoire | Rapid Vienna | 1999 | €5.0m |
| 6 |  Micael | Defender - Centre-Back | Brazil | Sociedade Esportiva Palmeiras | 2000 | €5.0m |
| 7 |  Malang Sarr | Defender - Centre-Back | France Senegal | RC Lens | 1999 | €5.0m |
| 8 |  Nahuel Ferraresi | Defender - Centre-Back | Venezuela Italy | São Paulo Futebol Clube | 1998 | €5.0m |
| 9 |  Moritz Jenz | Defender - Centre-Back | Germany Nigeria | VfL Wolfsburg | 1999 | €5.0m |
| 10 |  Ozan Kabak | Defender - Centre-Back | Türkiye | TSG 1899 Hoffenheim | 2000 | €5.0m |
| 11 |  Sebastian Walukiewicz | Defender - Centre-Back | Poland | US Sassuolo | 2000 | €4.5m |
| 12 |  Kamil Piatkowski | Defender - Centre-Back | Poland | Legia Warszawa | 2000 | €4.5m |
| 13 |  Liam Kitching | Defender - Centre-Back | England Ireland | Coventry City | 1999 | €4.5m |
| 14 |  Bruno Méndez | Defender - Centre-Back | Uruguay Spain | Deportivo Toluca | 1999 | €4.5m |
| 15 |  João Marcelo | Defender - Centre-Back | Brazil | Cruzeiro Esporte Clube | 2000 | €5.5m |
| 16 |  Emirhan Topçu | Defender - Centre-Back | Türkiye | Besiktas JK | 2000 | €5.5m |
| 17 |  Guzmán Rodríguez | Defender - Centre-Back | Uruguay Italy | Red Bull Bragantino | 2000 | €4.0m |
| 18 |  Wagner Leonardo | Defender - Centre-Back | Brazil | Grêmio Foot-Ball Porto Alegrense | 1999 | €4.0m |
| 19 |  Vanja Drkusic | Defender - Centre-Back | Slovenia | Zenit St. Petersburg | 1999 | €4.0m |
| 20 |  Francisco Álvarez | Defender - Centre-Back | Argentina | AA Argentinos Juniors | 2000 | €4.0m |
| 21 |  Mohamed Abdelmonem | Defender - Centre-Back | Egypt | OGC Nice | 1999 | €4.0m |
| 22 |  Moustapha Mbow | Defender - Centre-Back | Senegal | Paris FC | 2000 | €4.0m |
| 23 |  Christoph Klarer | Defender - Centre-Back | Austria | Birmingham City | 2000 | €4.0m |
| 24 |  Israel Reyes | Defender - Centre-Back | Mexico | CF América | 2000 | €4.0m |
| 25 |  Petko Hristov | Defender - Centre-Back | Bulgaria | Spezia Calcio | 1999 | €4.0m |
| 26 |  Joaquim | Defender - Centre-Back | Brazil | Tigres UANL | 1998 | €6.0m |
| 27 |  Jordan Beyer | Defender - Centre-Back | Germany | Burnley FC | 2000 | €6.0m |
| 28 |  Mark McKenzie | Defender - Centre-Back | United States Jamaica | FC Toulouse | 1999 | €6.0m |
| 29 |  Sebastiaan Bornauw | Defender - Centre-Back | Belgium | Leeds United | 1999 | €6.0m |
| 30 |  Gabriel Pereira | Defender - Centre-Back | Brazil | FC Copenhagen | 2000 | €6.0m |