| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Mario Hermoso | Defender - Centre-Back | Spain | AS Roma | 1995 | €7.0m |
| 2 |  Lucas Martínez Quarta | Defender - Centre-Back | Argentina Italy | CA River Plate | 1996 | €7.0m |
| 3 |  Yeray Álvarez | Defender - Centre-Back | Spain | Athletic Bilbao | 1995 | €7.0m |
| 4 |  Denis Vavro | Defender - Centre-Back | Slovakia | VfL Wolfsburg | 1996 | €7.0m |
| 5 |  Duje Caleta-Car | Defender - Centre-Back | Croatia | Real Sociedad | 1996 | €7.0m |
| 6 |  Clément Lenglet | Defender - Centre-Back | France | Atlético de Madrid | 1995 | €7.5m |
| 7 |  Jeremiah St. Juste | Defender - Centre-Back | Netherlands St. Kitts & Nevis | Sporting CP B | 1996 | €6.0m |
| 8 |  Omar Alderete | Defender - Centre-Back | Paraguay | Sunderland AFC | 1996 | €8.0m |
| 9 |  Niklas Süle | Defender - Centre-Back | Germany | Borussia Dortmund | 1995 | €8.0m |
| 10 |  Willyan Rocha | Defender - Centre-Back | Brazil Italy | Al-Jazira Club | 1995 | €6.0m |
| 11 |  Carl Starfelt | Defender - Centre-Back | Sweden | Celta de Vigo | 1995 | €6.0m |
| 12 |  Srdjan Babic | Defender - Centre-Back | Serbia Bosnia-Herzegovina | Spartak Moscow | 1996 | €6.0m |
| 13 |  Ardian Ismajli | Defender - Centre-Back | Albania Kosovo | Torino FC | 1996 | €6.0m |
| 14 |  Lukas Klostermann | Defender - Centre-Back | Germany | RB Leipzig | 1996 | €8.0m |
| 15 |  Martin Valjent | Defender - Centre-Back | Slovakia | RCD Mallorca | 1995 | €6.0m |
| 16 |  Nico Elvedi | Defender - Centre-Back | Switzerland | Borussia Mönchengladbach | 1996 | €8.0m |
| 17 |  Lucas Veríssimo | Defender - Centre-Back | Brazil | Al-Wakrah SC | 1995 | €5.5m |
| 18 |  Bernardo | Defender - Centre-Back | Brazil | TSG 1899 Hoffenheim | 1995 | €5.5m |
| 19 |  Léo Pereira | Defender - Centre-Back | Brazil | CR Flamengo | 1996 | €9.0m |
| 20 |  Presnel Kimpembe | Defender - Centre-Back | France DR Congo | Qatar SC | 1995 | €5.0m |
| 21 |  Alexander Barboza | Defender - Centre-Back | Argentina Uruguay | Botafogo de Futebol e Regatas | 1995 | €5.0m |
| 22 |  Abdou Diallo | Defender - Centre-Back | Senegal France | Al-Duhail SC | 1996 | €5.0m |
| 23 |  Jan Bednarek | Defender - Centre-Back | Poland | FC Porto | 1996 | €9.0m |
| 24 |  Kevin Diks | Defender - Centre-Back | Indonesia Netherlands | Borussia Mönchengladbach | 1996 | €5.0m |
| 25 |  Alan Franco | Defender - Centre-Back | Argentina | São Paulo Futebol Clube | 1996 | €4.5m |
| 26 |  Scott McKenna | Defender - Centre-Back | Scotland | GNK Dinamo Zagreb | 1996 | €4.0m |
| 27 |  Aleksandr Soldatenkov | Defender - Centre-Back | Russia | FC Sochi | 1996 | €4.0m |
| 28 |  Mason Holgate | Defender - Centre-Back | Jamaica England | Al-Gharafa SC | 1996 | €4.0m |
| 29 |  Sebastiano Luperto | Defender - Centre-Back | Italy | Cagliari Calcio | 1996 | €4.0m |
| 30 |  Niklas Stark | Defender - Centre-Back | Germany | SV Werder Bremen | 1995 | €4.0m |