| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Nico Schlotterbeck | Defender - Centre-Back | Germany | Borussia Dortmund | 1999 | €40.0m |
| 2 |  Matthijs de Ligt | Defender - Centre-Back | Netherlands | Manchester United | 1999 | €38.0m |
| 3 |  Sven Botman | Defender - Centre-Back | Netherlands | Newcastle United | 2000 | €42.0m |
| 4 |  Ronald Araujo | Defender - Centre-Back | Uruguay Spain | FC Barcelona | 1999 | €35.0m |
| 5 |  Edmond Tapsoba | Defender - Centre-Back | Burkina Faso | Bayer 04 Leverkusen | 1999 | €35.0m |
| 6 |  Jan Paul van Hecke | Defender - Centre-Back | Netherlands | Brighton & Hove Albion | 2000 | €32.0m |
| 7 |  Alessandro Buongiorno | Defender - Centre-Back | Italy | SSC Napoli | 1999 | €50.0m |
| 8 |  Isak Hien | Defender - Centre-Back | Sweden Ghana | Atalanta BC | 1999 | €30.0m |
| 9 |  Evan Ndicka | Defender - Centre-Back | Cote d'Ivoire France | AS Roma | 1999 | €30.0m |
| 10 |  Dayot Upamecano | Defender - Centre-Back | France Guinea-Bissau | Bayern Munich | 1998 | €50.0m |
| 11 |  Sam Beukema | Defender - Centre-Back | Netherlands | SSC Napoli | 1998 | €28.0m |
| 12 |  Jakub Kiwior | Defender - Centre-Back | Poland | FC Porto | 2000 | €28.0m |
| 13 |  Pierre Kalulu | Defender - Centre-Back | France DR Congo | Juventus FC | 2000 | €28.0m |
| 14 |  Toti | Defender - Centre-Back | Portugal Guinea-Bissau | Wolverhampton Wanderers | 1999 | €28.0m |
| 15 |  Hiroki Ito | Defender - Centre-Back | Japan | Bayern Munich | 1999 | €25.0m |
| 16 |  Calvin Bassey | Defender - Centre-Back | Nigeria England | Fulham FC | 1999 | €25.0m |
| 17 |  Jean-Clair Todibo | Defender - Centre-Back | France French Guiana | West Ham United | 1999 | €25.0m |
| 18 |  Trevoh Chalobah | Defender - Centre-Back | England Sierra Leone | Chelsea FC | 1999 | €25.0m |
| 19 |  Facundo Medina | Defender - Centre-Back | Argentina | Olympique Marseille | 1999 | €25.0m |
| 20 |  Kevin Danso | Defender - Centre-Back | Austria | Tottenham Hotspur | 1998 | €25.0m |
| 21 |  Loïc Badé | Defender - Centre-Back | France Cote d'Ivoire | Bayer 04 Leverkusen | 2000 | €25.0m |
| 22 |  Maxence Lacroix | Defender - Centre-Back | France Guadeloupe | Crystal Palace | 2000 | €25.0m |
| 23 |  Arthur Theate | Defender - Centre-Back | Belgium | Eintracht Frankfurt | 2000 | €24.0m |
| 24 |  Josip Sutalo | Defender - Centre-Back | Croatia | Ajax Amsterdam | 2000 | €24.0m |
| 25 |  Mohamed Simakan | Defender - Centre-Back | France Guinea | Al-Nassr FC | 2000 | €23.0m |
| 26 |  Aitor Paredes | Defender - Centre-Back | Spain | Athletic Bilbao | 2000 | €22.0m |
| 27 |  Leonardo Balerdi | Defender - Centre-Back | Argentina Italy | Olympique Marseille | 1999 | €20.0m |
| 28 |  Alexsandro | Defender - Centre-Back | Brazil | LOSC Lille | 1999 | €20.0m |
| 29 |  Ibrahima Konaté | Defender - Centre-Back | France Mali | Liverpool FC | 1999 | €60.0m |
| 30 |  Anel Ahmedhodzic | Defender - Centre-Back | Bosnia-Herzegovina Sweden | Feyenoord Rotterdam | 1999 | €18.0m |