| # | Player | Position | Citizenship | Club | Born | Value |
|---|---|---|---|---|---|---|
| 1 |  Leonardo Balerdi | Defender - Centre-Back | Argentina Italy | Olympique Marseille | 1999 | €20.0m |
| 2 |  Anel Ahmedhodzic | Defender - Centre-Back | Bosnia-Herzegovina Sweden | Feyenoord Rotterdam | 1999 | €18.0m |
| 3 |  Pascal Struijk | Defender - Centre-Back | Netherlands Belgium | Leeds United | 1999 | €18.0m |
| 4 |  Aitor Paredes | Defender - Centre-Back | Spain | Athletic Bilbao | 2000 | €22.0m |
| 5 |  Takehiro Tomiyasu | Defender - Centre-Back | Japan | Without Club | 1998 | €18.0m |
| 6 |  Oumar Solet | Defender - Centre-Back | France Cote d'Ivoire | Udinese Calcio | 2000 | €18.0m |
| 7 |  Jaka Bijol | Defender - Centre-Back | Slovenia | Leeds United | 1999 | €18.0m |
| 8 |  Diogo Leite | Defender - Centre-Back | Portugal | 1.FC Union Berlin | 1999 | €17.0m |
| 9 |  Roger Ibañez | Defender - Centre-Back | Brazil Italy | Al-Ahli SFC | 1998 | €17.0m |
| 10 |  Mohamed Simakan | Defender - Centre-Back | France Guinea | Al-Nassr FC | 2000 | €23.0m |
| 11 |  Lloyd Kelly | Defender - Centre-Back | England Jamaica | Juventus FC | 1998 | €16.0m |
| 12 |  Josip Sutalo | Defender - Centre-Back | Croatia | Ajax Amsterdam | 2000 | €24.0m |
| 13 |  Arthur Theate | Defender - Centre-Back | Belgium | Eintracht Frankfurt | 2000 | €24.0m |
| 14 |  Tuta | Defender - Centre-Back | Brazil | Al-Duhail SC | 1999 | €15.0m |
| 15 |  Maxence Lacroix | Defender - Centre-Back | France Guadeloupe | Crystal Palace | 2000 | €25.0m |
| 16 |  Jean-Clair Todibo | Defender - Centre-Back | France French Guiana | West Ham United | 1999 | €25.0m |
| 17 |  Calvin Bassey | Defender - Centre-Back | Nigeria England | Fulham FC | 1999 | €25.0m |
| 18 |  Facundo Medina | Defender - Centre-Back | Argentina | Olympique Marseille | 1999 | €25.0m |
| 19 |  Hiroki Ito | Defender - Centre-Back | Japan | Bayern Munich | 1999 | €25.0m |
| 20 |  Loïc Badé | Defender - Centre-Back | France Cote d'Ivoire | Bayer 04 Leverkusen | 2000 | €25.0m |
| 21 |  Trevoh Chalobah | Defender - Centre-Back | England Sierra Leone | Chelsea FC | 1999 | €25.0m |
| 22 |  Kevin Danso | Defender - Centre-Back | Austria | Tottenham Hotspur | 1998 | €25.0m |
| 23 |  Chris Richards | Defender - Centre-Back | United States | Crystal Palace | 2000 | €14.0m |
| 24 |  Johan Vásquez | Defender - Centre-Back | Mexico | Genoa CFC | 1998 | €14.0m |
| 25 |  Dara O'Shea | Defender - Centre-Back | Ireland | Ipswich Town | 1999 | €14.0m |
| 26 |  Matteo Gabbia | Defender - Centre-Back | Italy | AC Milan | 1999 | €14.0m |
| 27 |  Nehuén Pérez | Defender - Centre-Back | Argentina | FC Porto | 2000 | €13.0m |
| 28 |  Vitão | Defender - Centre-Back | Brazil | Sport Club Internacional | 2000 | €12.0m |
| 29 |  Mohammed Salisu | Defender - Centre-Back | Ghana | AS Monaco | 1999 | €12.0m |
| 30 |  Youssouf Ndayishimiye | Defender - Centre-Back | Burundi | OGC Nice | 1998 | €12.0m |